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human c4 protein  (Quidel)


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    Structured Review

    Quidel human c4 protein
    Human C4 Protein, supplied by Quidel, used in various techniques. Bioz Stars score: 95/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+c4+protein/C4+Protein/pmc12344723-671-29-32
    Average 95 stars, based on 10 article reviews
    human c4 protein - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Purification:

    Article Title: Schizophrenia risk from complex variation of complement component 4
    Article Snippet: .. For pre-adsorption experiments, purified human C4 protein (Quidel) was pre-incubated with the C4c antibody at double the antibody concentration for 30 minutes at room temperature before being added to the slides for overnight incubation at 4oC. ..

    Article Title: Schizophrenia risk from complex variation of complement component 4
    Article Snippet: Equal amounts of proteins (20 ug as determined by BCA Protein Assay) were diluted 1:1 with Native Sample Buffer (BioRad 161-0738) and separated on a 4-15% TGX precast polyacrylamide gel. .. Purified human C4 protein from Quidel (A402) was used as a positive control. .. Unconditioned neuronal media (Sciencell 1521) provided an appropriate negative control.

    Concentration Assay:

    Article Title: Schizophrenia risk from complex variation of complement component 4
    Article Snippet: .. For pre-adsorption experiments, purified human C4 protein (Quidel) was pre-incubated with the C4c antibody at double the antibody concentration for 30 minutes at room temperature before being added to the slides for overnight incubation at 4oC. ..

    Incubation:

    Article Title: Schizophrenia risk from complex variation of complement component 4
    Article Snippet: .. For pre-adsorption experiments, purified human C4 protein (Quidel) was pre-incubated with the C4c antibody at double the antibody concentration for 30 minutes at room temperature before being added to the slides for overnight incubation at 4oC. ..

    Positive Control:

    Article Title: Schizophrenia risk from complex variation of complement component 4
    Article Snippet: Equal amounts of proteins (20 ug as determined by BCA Protein Assay) were diluted 1:1 with Native Sample Buffer (BioRad 161-0738) and separated on a 4-15% TGX precast polyacrylamide gel. .. Purified human C4 protein from Quidel (A402) was used as a positive control. .. Unconditioned neuronal media (Sciencell 1521) provided an appropriate negative control.



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    Image Search Results


    Antibodies used for flow cytometry.

    Journal: Frontiers in Oncology

    Article Title: Establishment, characterization, and drug screening of low-passage patient individual non-small cell lung cancer in vitro models including the rare pleomorphic subentity

    doi: 10.3389/fonc.2023.1089681

    Figure Lengend Snippet: Antibodies used for flow cytometry.

    Article Snippet: LYPD3 , APC , Sino Biological Europe GmbH, Düsseldorf, Germany , 11836-H08H.

    Techniques: Cytometry

    Flow cytometry. Histogram overlays of unstained controls (dotted lines for all three cell lines) and measurements for HROLu55 (green), HROLu22 (blue), and HROBML01 (red) for the epitopes CD326, PD-L1, EGFR, CD26, LYPD3, DSG3, CCD59, CD27, and CD90 are shown.

    Journal: Frontiers in Oncology

    Article Title: Establishment, characterization, and drug screening of low-passage patient individual non-small cell lung cancer in vitro models including the rare pleomorphic subentity

    doi: 10.3389/fonc.2023.1089681

    Figure Lengend Snippet: Flow cytometry. Histogram overlays of unstained controls (dotted lines for all three cell lines) and measurements for HROLu55 (green), HROLu22 (blue), and HROBML01 (red) for the epitopes CD326, PD-L1, EGFR, CD26, LYPD3, DSG3, CCD59, CD27, and CD90 are shown.

    Article Snippet: LYPD3 , APC , Sino Biological Europe GmbH, Düsseldorf, Germany , 11836-H08H.

    Techniques: Flow Cytometry

    Antibodies used for flow cytometry.

    Journal: Frontiers in Oncology

    Article Title: Establishment, characterization, and drug screening of low-passage patient individual non-small cell lung cancer in vitro models including the rare pleomorphic subentity

    doi: 10.3389/fonc.2023.1089681

    Figure Lengend Snippet: Antibodies used for flow cytometry.

    Article Snippet: LYPD3 , APC , Sino Biological Europe GmbH, Düsseldorf, Germany , 11836-H08H.

    Techniques: Cytometry

    Antibodies used for flow cytometry.

    Journal: Frontiers in Oncology

    Article Title: Establishment, characterization, and drug screening of low-passage patient individual non-small cell lung cancer in vitro models including the rare pleomorphic subentity

    doi: 10.3389/fonc.2023.1089681

    Figure Lengend Snippet: Antibodies used for flow cytometry.

    Article Snippet: LYPD3 , APC , Sino Biological Europe GmbH, Düsseldorf, Germany , 11836-H08H.

    Techniques: Cytometry

    AACT exosomal levels in dementia and AD cases monitored by distinct antibody-based approaches. AACT levels were assessed through immunoblot analysis or commercial ELISA assays in serum-derived exosomes from Controls (CDR = 0 and MMSE −) and individuals with dementia (CDR ≥ 1 and MMSE +) from UA-cohort ( a , d ), and AD clinically diagnosed cases from UA-cohort ( b , e ) or UMG-cohort ( c , f ). For WB, each point represents the relative densitometry ratio. For ELISA, each point represents the mean concentration value obtained for each individual. The solid horizontal line shows mean, and error bars indicates standard deviations. Abbreviations: AD, Alzheimer’s disease; C, Controls; CDR, Clinical Dementia Rate; MMSE, Mini-Mental State Examination. * p ≤ 0.05

    Journal: Molecular Neurobiology

    Article Title: Novel Exosome Biomarker Candidates for Alzheimer’s Disease Unravelled Through Mass Spectrometry Analysis

    doi: 10.1007/s12035-022-02762-1

    Figure Lengend Snippet: AACT exosomal levels in dementia and AD cases monitored by distinct antibody-based approaches. AACT levels were assessed through immunoblot analysis or commercial ELISA assays in serum-derived exosomes from Controls (CDR = 0 and MMSE −) and individuals with dementia (CDR ≥ 1 and MMSE +) from UA-cohort ( a , d ), and AD clinically diagnosed cases from UA-cohort ( b , e ) or UMG-cohort ( c , f ). For WB, each point represents the relative densitometry ratio. For ELISA, each point represents the mean concentration value obtained for each individual. The solid horizontal line shows mean, and error bars indicates standard deviations. Abbreviations: AD, Alzheimer’s disease; C, Controls; CDR, Clinical Dementia Rate; MMSE, Mini-Mental State Examination. * p ≤ 0.05

    Article Snippet: Further, AACT and C4BPα levels were also evaluated by enzyme-linked immunosorbent assay (ELISA), in serum-derived exosomes of the same individuals, using the commercial Human AACT ELISA Kit (ab217779; Abcam) or the Human C4 binding protein A ELISA Kit (NBP2-60,550; Novus Biologicals), according to manufacturer’s instructions.

    Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Derivative Assay, Concentration Assay